Multiple space and time scales arise in plasma turbulence in magnetic confinement fusion devices because of the smallness of the square root of the electron-to-ion mass ratio and the consequent disparity of the ion and electron thermal gyroradii and thermal speeds. Direct simulations of this turbulence that include both ion and electron space-time scales indicate that there can be significant interactions between the two scales. The extreme computational expense and complexity of these direct simulations motivates the desire for reduced treatment. By expanding in sqrt(m_e /m_i ) we derive such a reduced system of gyrokinetic equations that describes cross-scale interactions by exploiting the scale separation between ion and electron scales. The coupled gyrokinetic equations contain novel terms which provide candidate mechanisms for the observed cross-scale interaction. The electron scale turbulence experiences a modified drive due to gradients in the ion scale distribution function, and is advected by the ion scale E ∧ B drift, which varies in the direction parallel to the magnetic field line. The largest possible cross-scale term in the ion scale equations is sub-dominant in our sqrt(m_e /m_i ) expansion. Hence, in our model the ion scale turbulence evolves independently of the electron scale turbulence. To complete the scale-separated approach, we provide and justify a parallel boundary condition for the coupled gyrokinetic equations in axisymmetric equilibria based on the standard “twist-and-shift” boundary condition. This approach allows one to simulate multi-scale turbulence using electron scale flux tubes nested within an ion scale flux tube.